Cleanroom system for production of human hepatocytes from a mouse model

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Challenge: Create a Class 1000 – Class 100 cleanroom for cell production within a room not originally designed for lab work.

Solution: Create a freestanding, portable, positive pressure cleanroom that operates at 50 HEPA filtered air changes per hour.

Three separate areas make up the cleanroom system:

  • Anteroom for sterile PPE storage and donning and doffing of PPE
  • Clean procedure space for perfusion of hepatocytes from the mouse model
  • Cleanroom for cell culture

The bioBUBBLE system is designed with the cleanest HEPA filtered air starting in the cell culture space and flowing through the procedure space and then the anteroom.