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AALAS 66th National Meeting

Phoenix, AZ

Platform Session- Husbandry Management 3

8:00 AM – 10:30 AM / Room 124A

November 4, 2015

Abdel Ayadi

Institut Clinique de la Souris, Illkirch France

PS48 Decontamination of Valuable Stocks in a Barrier Facility Using Embryo Transfer Rederivation Procedures in a Class 100 Environment

A Ayadi*, I Goncalves, D Ali-Hadji, S Legeay, P Charles, P Andr., C Kujath, T Sorg, H Yann

Institut Clinique de la Souris, Universit. de Strasbourg, Illkirch, France

The number of genetically engineered mice models (GEMs) is growing rapidly due to high-throughput production strategies employed by the International Mouse Phenotyping Consortium (IMPC). Mouse repositories play an important role in promoting and distributing the models produced by such a large international project. The microbiological quality of these distributed models is critical as health status can alter animal welfare, validity and reproducibility of research data. As one of these mouse repositories, we faced endemic infection with opportunistic agents (Helicobacter, Pasteurella and Norovirus) that represent importation issues for researchers requesting strains from our collection. The solution to get rid of these undesired agents is to conduct embryo transfer rederivation procedures. Typically, decontaminations are performed in a different barrier with a higher health status.

Isolators within the parent facility are a viable alternative as they provide an elevated level of animal protection from surrounding environment. However isolators are operationally intensive, and not convenient for mice procedures. Clean rooms operating under positive pressure using HEPA filtration to provide protection for both housing and procedural areas, represent an option that is less labor intensive, and reducing operating costs. We report here the outcome of a 24-month program to rederive GEMs internally with a customized class 100 environment. This enclosure has been installed within our contaminated facility. Embryo-recipient females and rederived mice were health-screened to assess their microbiological status according to the FELASA guidelines. More than 50 strains so far has been successfully rederived free of Helicobacter, Pasteurella and Norovirus, the 3 agents endemically present in our facility with a high prevalence (76, 21 and 94% respectively). To date, 24 months of follow-up mice screening indicates that we achieved our goal of eradicating endemic infectious agents, for strains to be distributed from our repository, based on the use of clean room enclosure.

Besides the outcome of this rederivation program we also present a time/cost analysis between solutions to upgrade mice health status, which drive us in these times of financial limitations.

Learn more about this project.

Visit bioBUBBLE Representatives at Booth #547 if you have any questions.